Mandatory and recommended fields for sharing data with GGBN

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Please contact us via email (support@ggbn.org) if you have any questions related to sharing your data with GGBN.


Introduction

ABCD and DarwinCore both contain hundreds of elements. Some are mandatory. The curator of the DNA Bank has to define the Metadata of the DNA Bank collection first. After that the real mapping can begin. You can use BioCASe or IPT to provide data to GGBN, but have to make sure you use the extensions for the GGBN Data Standard. DarwinCore and ABCD have a lot of terms in common, but use different names for the terms. Therefore the following section are splitted into ABCD and DwC. GGBN supports both standards, both have pros and cons and work well for GGBN. It depends on your data and if your institution is already providing data to GBIF which one fits better. Please contact us if you need help.

Asterisk (*) means mandatory for GGBN, all others are recommended.

Separate mappings for voucher specimens (mapping 1) and tissue and DNA samples (mapping 2) are preferred. This enables third parties such as GBIF or GGBN to harvest only those data that are relevant.

All metadata information should be in English! Please map as many elements as possible, the better your data visible and searchable in GBIF and GGBN the better for other scientists and at the end for yourself!

Mapping principles

You can provide your data to GGBN in different ways. It depends on your other collections and the way you provide your data already to GBIF which one fits best for you. GGBN can handle all of the examples below, but it might make a difference for your occurrences at GBIF. Most important is, that you provide the references between the records in a standardized way. DNA collections comprise many difference use cases. Not in all cases you will have a physicial tissue or a physicial specimen. Please contact us if you need help.

Mapping with Specimens

A specimen in terms of GGBN and GBIF is a preserved specimen from wild origin or a living specimen (cultivated plant, captivated animal, cell culture). In case your collection or certain DNA samples have no corresponding tissue sample, you should refer to the "mother" specimen directly.

Specimens are often deposited in external collections. If you provide the proper identifiers the GGBN Data Portal can aggregate this information! We know that it is impossible to track this information back for all legacy data. But if you keep this in mind in your daily workflows for all new records and samples the quality and value of your data and collection will increase enormously.
Mapping all at once Mapping two sources Mapping three sources
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Pros: - only one mapping needed

Cons: - double occurrences at GBIF, since GBIF can't handle associataions

Pros: - Only underlying specimens records are provided, no double entries at GBIF

Cons: - two mappings needed (but can be easily copied)

Pros: - Only underlying specimens records are provided, no double entries at GBIF

- Clear distinction between collections, some managers prefer this mapping type

Cons: - three mappings needed (but can be easily copied)

Mapping without Specimens

Often tissue samples are collected without collecting/killing the whole organism. There are two ways to handle this.

Mapping eVouchers Mapping tissues only
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In case you want to track an individual, sampled multiple times (e.g. vertebrates catched multiple times and marked individually, trees) you should add an Observation record as top level. This could also be accompanied by images, then called eVoucher. In case you don't want to track individuals, just provide your tissue records to GBIF as top level occurrence.

ABCD

Metadata of your DNA bank

Metadata are very important. They will be displayed with every single DNA record in the portal. Be careful with the IPR statements!

Group Element Remarks Example
ContentContact *Address Complete Address of the responsible person Botanic Garden and Botanical Museum Berlin-Dahlem, Freie Universität Berlin, Koenigin-Luise-Str. 6-8, 14195 Berlin, Germany
ContentContact *Email valid email address; will be used by the web portal for sending you annotations etc. please use @ instead of masquerades like "[at]"
ContentContact *Name Person, person team responsible for curation of your DNA Bank Gabi Droege
Description/Representation *Details Short text to describe the focus and number of samples in your DNA collection. It should include the following phrase: The DNA bank is part of the Global Genome Biodiversity Network (GGBN) which was founded in 2011. The network provides a technically optimized DNA collection service facility for all biological research accessible via one central web portal. The network promotes deposition of well documented reference DNA samples after project completion or data publication from scientists of other universities and institutions. The DNA bank of the Botanic Garden and Botanical Museum Berlin-Dahlem holds currently a collection of 20000 plant DNA and tissue samples growing constantly. Its core collection focuses on the flora of Germany but it comprises botanical samples collected worldwide.
Description/Representation *@language language of description should be "en" as literal
Description/Representation *Title Short title that describes your DNA collection DNA Bank of the Herbarium Berolinense
IPRStatements/Citation *Text Droege, G. (Ed.) 2008 - (continuously updated): DNA samples of the DNA bank at the BGBM (Botanic Garden and Botanical Museum Berlin-Dahlem).
IPRStatements/Citation *@language language of citation should be "en" as literal
IPRStatements/Copyright *Text The copyright for any material created by the DNA bank of the BGBM is reserved. The duplication or use of information and data such as texts or images is only permitted with the indication of the source or with prior approval by the BGBM.
IPRStatements/Copyright *@language language of copyright should be "en" as literal
IPRStatements/TermsOfUse *Text The use of the data is allowed only for non-profit scientific use and for non-profit nature conservation purpose. The data bases or part of it may only be used or copied by the written permission from the legal owner.
IPRStatements/TermsOfUse *@language language of the terms of use should be "en" as literal
IconURI complete url path to the logo of your institution
Owner *Address Complete Address of the institution that owns the DNA bank samples and data
Owner/Representation *Text name of your institution Botanic Garden an Botanical Museum Berlin-Dahlem (Freie Universität Berlin)
Owner *URL path to website of your institution http://www.bgbm.org
RevisionData *DateModified date of last modification of your data
TechnicalContact *Address Complete Address of the responsible person Botanic Garden and Botanical Museum Berlin-Dahlem, Freie Universität Berlin, Koenigin-Luise-Str. 6-8, 14195 Berlin, Germany
TechnicalContact *Email valid email address; will be used by the web portal for sending you annotations etc. please use @ instead of masquerades like "[at]"
TechnicalContact *Name Person, person team responsible for technical issues of your database Gabriele Droege
Unit *RecordBasis this value must be part of the ABCD vocabulary must be "MaterialSample"
Unit *KindOfUnit the type of sample e.g. DNA

Related Specimen Data

All specimen voucher information must be available via a GBIF compliant database! Both Darwin Core-Archive and BioCASE specimen providers are possibe! To provide the underlying specimen data you can use your existing GBIF dataset mapping.
In the database and the mapping the child record should refer to its mother record in opposite to the real world example

The voucher identifiers

Group Element Remarks Example
Associations/UnitAssociation *UnitID the UnitID or CatalogueNumber used for GBIF e.g. the barcode number of your specimens
Associations/UnitAssociation *SourceInstitutionID the SourceInstitutionID or InstitutionCode used for GBIF e.g. the acronym of your institution
Associations/UnitAssociation *SourceName the SourceID or CollectionCode used for GBIF e.g. the name of the collection where the specimen belongs to, e.g. "Birds"
Associations/UnitAssociation *AssociationType the Relation between the DNA and the voucher e.g. "DNA and voucher from same individual"
Associations/UnitAssociation *DatasetAccessPoint the wrapper url of the voucher record e.g. "http://ww3.bgbm.org/biocase/pywrapper.cgi?dsa=Herbar"

Gathering event of the voucher

All elements marked with * will be indexed and must be mapped! Mapping these gathering facts twice (one for the specimen database and one for the DNA mapping) is required because of indexing and later search purposes.

Group Element Remarks Example
*CollectorsFieldNumber the number the collector gave to the specimen in the field, often used in Botany but not in Zoology; map it if you have content e.g. 765/10
Gathering/Agents/GatheringAgent *FullName the Collector or Collector Team e.g. Scholz & Sipman
Gathering/Altitude *LowerValue if you have both the lower and upper value in different columns map both field, if not map LowerValue only e.g. 100
Gathering/Altitude UpperValue e.g. 200
Gathering/Altitude *Unit e.g. m
Gathering/Country *ISO3166Code ISO code of the country where the voucher was collected e.g. US
Gathering/Country *Name english Name of the country e.g. United States of America
Gathering/DateTime *DateText date when voucher was collected, if you have content yuo can also use ISO format e.g. 21. April 1951
Gathering/Locality *LocalityText e.g. 5km NO Berlin
Gathering/NamedArea AreaName name of continent e.g. Europe
Gathering/NamedArea @language language of the name of continent e.g. "en"
Gathering/SiteCoordinates *LatitudeDecimal e.g. -15,88876
Gathering/SiteCoordinates *LongitudeDecimal e.g. 72,88876

Identification history of the voucher

Mostly specimen databases record the complete determination or identification history of a single specimen. Please note that DiGIR/DarwinCore is not able to deal with more than one determination! In opposite ABCD has many repeatable elements and can handle multiple determinations. For the DNA Bank Network we try to get all available information into the portal. So if you use DiGIR for your specimens you might think about switching to ABCD and BioCASE instead. It's the same with Multimedia urls. DarwinCore can provide only one url, ABCD provides as much urls as required.

Group Element Remarks Example
Identification *PreferredFlag mark the presently preferred Identification e.g. true, false, 0, 1
Identification/HigherTaxon *HigherTaxonName the name of the higher taxon, please have a look at the BioCASE Wiki for how to prepare your database for the repeatable elements e.g. Asteraceae, Animalia
Identification/HigherTaxon *HigherTaxonRank the rank of the taxon in english or latin e.g. familia, regnum, phylum
Identification/ScientificName *FullScientificName the complete name of the taxon including Authors (and years for animals) e.g. Aaronsohnia factorovskyi Warb. & Eig. var. factorovskyi
Identification/ScientificName/NameAtomised *FirstEpithet Please note: ABCD has several container for NameAtomised, it depens on your sampes which one to choose (Botanical or Zoology etc.) e.g. factorovskyi
Identification/ScientificName/NameAtomised *GenusOrMonomial e.g. Aaronsohnia
Identification/ScientificName/NameAtomised *InfraspecificEpithet Please note: ABCD has several container for NameAtomised, it depens on your sampes which one to choose (Botanical or Zoology etc.) e.g. factorovskyi
Identification/ScientificName/NameAtomised *Rank Please note: ABCD has several container for NameAtomised, it depens on your sampes which one to choose (Botanical or Zoology etc.) e.g. var.

Multimedia items of the voucher

These should be mapped in the specimen mapping, not the DNA mapping.

DNA sample data

The DNA identifier

We use ABCD identifier the same way as GBIF does. Please note that UnitID should be unique in one database! Mostly the extraction number is used for it.

Group Element Remarks Example
Unit *SourceID short description of relevant collection should be "DNA Bank"
Unit *SourceInstitutionID short name/abbreviation of relevant institution BGBM
Unit *UnitID DNA extraction number DNA 123

The DNA extension

The DNA extension contains much more elements than presently in use. When we developed the schema we wanted to propose a standard for exchange of molecular data in general. The following fields are the most common in use, but feel free to map more!

Group Element Remarks Example
DNA/Amplification AmplificationDate date of amplification; if unknown or general without content you don't have to map it should be ISO format yyyy-mm-dd
DNA/Amplification *GeneticLocus COX1
DNA/Amplification *GenBankNumber the accession number of NCBI/EMBL/DDBJ or the process ID of BOLD; this is a repeatable element, you can provide as much as you want e.g. AJ45567
DNA/Amplification *GenBankNumber-URI complete link to the accession number of NCBI/EMBL/DDBJ or the process ID of BOLD
DNA/Amplification ReferenceCitation molecular publication related to this DNA sample Citation format, free text
DNA/Amplification ReferenceURI if available link to online version
DNA *BlockedUntil in case the DNA sample is blocked until a specific date it's nevertheless searchable but customers cannot order it ISO format
DNA *Concentration map only if filled with content; concentration of the DNA 65,34
DNA *@Unit unit of the concentration µg/ml
DNA *ExtractionDate date of DNA extraction if unknown type "unknown"
DNA *ExtractionMethod extraction kit or protocoll if unknown type "unknown"
DNA *ExtractionStaff extraction staff if unknown type "unknown"
DNA *Preservation preservation of the tissue if unknown type "unknown"
DNA ProvidedBy if someone provided the samples
DNA PurificationMethod purification kit or protocoll
DNA Quality quality of the sample high, medium, low
DNA *QualityCheckDate date when quality parameters has been measured, e.g. Nanodrop etc. ISO format
DNA *RatioOfAbsorbance260_230 map only if filled with content 1,2
DNA *RatioOfAbsorbance260_280 map only if filled with content 1,8
DNA *Tissue type of tissue used for DNA extraction e.g. leaf, blood, leg
Unit Notes Notes for the customer